Culture media

200 ml of 100% Z8 diluted in 800 ml of GF/C filtered seawater ( ca 32-34 PSU), autoclaved. 

L1 (L1-Si, L1-Si+NH4)

The L1-medium is a modification of the f/2 medium. The difference is a broader trace metal composition in L1. We use it as standard medium for marine diatoms. The L1-trace metal solution is used also in many other media.

This medium is used for Dunaliella salina.

We have used MV (Marine medium with Vitamins) for many years as a standard medium for marine benthic algae. This is the medium named ESI by Tatewaki (1966) but supplemented with vitamins.

This medium is a modification of an Erd-Schreiber medium.

This medium was originally formulated for growth of Peridinium gatunense from Lake Kinneret (Lindström 1991), but works very well for other dinoflagellates as well.

We use the recipe in Guillard et al. (1972), but often use a slight modification according to Rengefors (pers. com.): TES- is used instead of TRIS buffer and selenium is added.

This medium is a slight modification of the Bs medium (Christensen 1982). Bs is a modification of the classic freshwater media of Bold and Bristol.

This medium (O stands for organic nutrient mixture) is useful for bacteria eating algae like Ochromonas viridis.

This is our standard medium for blue-green algae.